Goat anti-PGK1 Antibody |
| Principle Name | PGK1 |
| Official Symbol | PGK1 |
| All Name and Symbols | PGK1; phosphoglycerate kinase 1; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; primer recognition protein 2 |
| Accession Number(s) | NP_000282.1 |
| Peptide Sequence | SKKYAEAVTRAKQ, is from internal region |
| Human Gene ID(s) | 5230 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 45kDa band observed in lysates of cell lines A432, HEK293, HeLa and HepG2 (calculated MW of 44.6kDa according to NP_000282.1). Recommended concentration |
| Reference(s) | Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, Pienta KJ, Taichman RS. Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression. Cancer research 2010 Jan 70 (2) |
