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Goat anti-Pro-EGF Antibody
Item#:
dAP-3100
Size Price Quantity
50.0 ug $310.00
Goat anti-Pro-EGF Antibody
Principle Name Pro-EGF
Official Symbol EGF
All Name and Symbols EGF; epidermal growth factor; HOMG4; URG; OTTHUMP00000162922; OTTHUMP00000219721; OTTHUMP00000219722; beta-urogastrone; pro-epidermal growth factor
Accession Number(s) NP_001954.2; NP_001171601.1; NP_001171602.1
Peptide Sequence SRQERVCNIEKNVS, is from internal region (near the N terminus)
Human Gene ID(s) 1950
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration
Reference(s) Araújo AP, Costa BM, Pinto-Correia AL, Fragoso M, Ferreira P, Dinis-Ribeiro M, Costa S, Reis RM, Medeiros R. Association between EGF +61A/G polymorphism and gastric cancer in Caucasians. World J Gastroenterol. 2011 Jan 28;17(4)

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