Goat anti-VRK2 Antibody |
| Principle Name | VRK2 |
| Official Symbol | VRK2 |
| All Name and Symbols | VRK2; vaccinia related kinase 2; vaccinia virus B1R-related kinase 2; vaccinia-related kinase-2 |
| Accession Number(s) | NP_001129499.1; NP_006287.2; NP_001123954.1; NP_001123955.1 |
| Peptide Sequence | PNSQKVDSQKAATK, is from internal region |
| Human Gene ID(s) | 7444 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | Approx 60kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1kDa according to NP_001123954.1). Recommended concentration |
| Reference(s) | Sanz-García M, López-Sánchez I, Lazo PA. Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Molecular & cellular proteomics |