Goat anti-CAPG Antibody |
| Principle Name | CAPG |
| Official Symbol | CAPG |
| All Name and Symbols | CAPG; capping protein (actin filament), gelsolin-like; AFCP; MCP; actin regulatory protein CAP-G; actin-regulatory protein CAP-G; gelsolin-like capping protein; macrophage capping protein; macrophage-capping protein |
| Accession Number(s) | NP_001738.2; NP_001243069.1 |
| Peptide Sequence | NIRATERALNWDS, is from internal region |
| Human Gene ID(s) | 822 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 38kDa band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration |
| Reference(s) | Hubert T, Van Impe K, Vandekerckhove J, Gettemans J. The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle. Biochemical and biophysical research communications 2009 Feb 380 (1) |
