Goat anti-CD25 / IL2RA (aa117-129) Antibody

Item#:

dAP-3083

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CD25 / IL2RA (aa117-129) Antibody
Principle Name	CD25 / IL2RA (aa117-129)
Official Symbol	IL2RA
All Name and Symbols	IL2RA; interleukin 2 receptor, alpha; CD25; IDDM10; IL2R; TCGFR; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; OTTHUMP00000019030; OTTHUMP00000019031; OTTHUMP00000019032; TAC antigen; interleukin-2 receptor subunit alpha; p55
Accession Number(s)	NP_000408.1
Peptide Sequence	DQASLPGHCREPP, is from internal region
Human Gene ID(s)	3559
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments in Human Colorectal cancer, Cerebellum, Heart and CACO-2 lysates gave no specific signal but low background (at antibody concentration up to 2µg/ml). We would appreciate any feedback from people in the field - have a
Reference(s)	Amu S, Brisslert M. Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin Dev Immunol. 2011;2011