Goat anti-ATRX Antibody |
| Principle Name | ATRX |
| Official Symbol | ATRX |
| All Name and Symbols | ATRX; alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae); RP5-875J14.1; ATR2; MGC2094; MRXS3; RAD54; RAD54L; SHS; XH2; XNP; ZNF-HX ; DNA dependent ATPase and helicase; OTTHUMP00000062079; RAD54 (Saccharomyces cerevisiae) |
| Accession Number(s) | NP_000480.2; NP_612114.1; NP_612115.1 |
| Peptide Sequence | KKDQSDETSEDDKKQ, is from internal region |
| Human Gene ID(s) | 546 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (NP_000480.2, NP_612114.1, NP_612115.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the expected size of 300kDa according to the literature (PMID |
| Reference(s) | Abidi FE, Cardoso C, Lossi AM, Lowry RB, Depetris D, Mattei MG, Lubs HA, Stevenson RE, Fontes M, Chudley AE, Schwartz CE. Mutation in the 5' alternatively spliced region of the XNP/ATR-X gene causes Chudley-Lowry syndrome. Eur J Hum Genet. 2005 Feb;13(2) |
