Goat anti-calreticulin Antibody

Item#:

dAP-3078

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-calreticulin Antibody
Principle Name	calreticulin
Official Symbol	CALR
All Name and Symbols	CALR; calreticulin; CRT; RO; SSA; cC1qR; CRP55; ERp60; HACBP; Sicca syndrome antigen A (autoantigen Ro; calreticulin); calregulin; endoplasmic reticulum resident protein 60; grp60
Accession Number(s)	NP_004334.1
Peptide Sequence	HPEIDNPEYSPDPS, is from internal region
Human Gene ID(s)	811
Non-Human GeneID(s)	12317 (mouse); 64202 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 60kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1kDa according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14;270(28)
Reference(s)	Jeffery E, Peters LR, Raghavan M. The polypeptide binding conformation of calreticulin facilitates its cell-surface expression under conditions of endoplasmic reticulum stress. The Journal of biological chemistry 2011 Jan 286 (4)