Goat anti-JUND Antibody |
| Principle Name | JUND |
| Official Symbol | JUND |
| All Name and Symbols | JUND; jun D proto-oncogene ; JunD-FL isoform; transcription factor jun-D |
| Accession Number(s) | NP_005345.3 |
| Peptide Sequence | CQLLPQHQVPAY, is from C Terminus |
| Human Gene ID(s) | 3727 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected NOT to cross-react with Jun oncogen (NP_002219.1, GeneID 3725) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 40kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 35.2kDa according to NP_005345.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Short and Pfarr,J |
| Reference(s) | Hartman TR, Qian S, Bolinger C, Fernandez S, Schoenberg DR, Boris-Lawrie K. RNA helicase A is necessary for translation of selected messenger RNAs. Nat Struct Mol Biol. 2006 Jun;13(6) |