Goat anti-HIF3A Antibody |
| Principle Name | HIF3A |
| Official Symbol | HIF3A |
| All Name and Symbols | HIF3A; hypoxia inducible factor 3, alpha subunit; HIF-3A; HIF-3A2; HIF-3A4; IPAS; MOP7; PASD7; bHLHe17; hypoxia-inducible factor-3 alpha 4; inhibitory PAS domain protein |
| Accession Number(s) | NP_690007.1; NP_690008.2 |
| Peptide Sequence | QDHRSTTELRKEKS, is from N Terminus |
| Human Gene ID(s) | 64344 |
| Non-Human GeneID(s) | 53417 (mouse); 64345 (rat); |
| Important Comments | This antibody is expected to recognize isoform a and c (NP_690007.1; NP_690008.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Immunohistochemistry | In paraffin embedded Human Kidney shows nuclear staining in some of the DCT. Recommended concentration, 4-6µg/ml. |
| Reference(s) | Tanaka T, Wiesener M, Bernhardt W, Eckardt KU, Warnecke C, The human HIF (hypoxia-inducible factor)-3alpha gene is a HIF-1 target gene and may modulate hypoxic gene induction. The Biochemical journal 2009 Nov 424 (1) |
