Goat anti-keratin 18 Antibody

Item#:

dAP-3042

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-keratin 18 Antibody
Principle Name	keratin 18
Official Symbol	KRT18
All Name and Symbols	KRT18; keratin 18; CYK18; K18; CK-18; cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; cytokeratin 18; cytokeratin-18; keratin, type I cytoskeletal 18; keratin-18
Accession Number(s)	NP_000215.1
Peptide Sequence	EELDKYWSQQIE, is from internal region
Human Gene ID(s)	3875
Non-Human GeneID(s)	16668 (mouse); 294853 (rat);
Important Comments	Reported variants represent identical protein
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Pig
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 48kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kDa according to NP_000215.1) and approx 45kDa in lysates of Pig Liver (calculated MW of 47.4kDa according to XP_005652636.2). Recommended concentrat
Reference(s)	Meng Y, Wu Z, Yin X, Zhao Y, Chen M, Si Y, Yang J, Fu X, Han W. Keratin 18 attenuates estrogen receptor alpha-mediated signaling by sequestering LRP16 in cytoplasm. BMC cell biology 2009 10