Goat anti-POLRMT Antibody

Item#:

dAP-3036

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-POLRMT Antibody
Principle Name	POLRMT
Official Symbol	POLRMT
All Name and Symbols	POLRMT; polymerase (RNA) mitochondrial (DNA directed); APOLMT; MTRNAP; MTRPOL; h-mtRPOL; DNA-directed RNA polymerase, mitochondrial
Accession Number(s)	NP_005026.3
Peptide Sequence	KETLQAVPKPGAFD, is from C Terminus
Human Gene ID(s)	5442
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Preliminary experiments gave bands at approx 90kDa and 35kDa in Human HepG2 lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated siz
Reference(s)	Ringel R, Sologub M, Morozov YI, Litonin D, Cramer P, Temiakov D. Structure of human mitochondrial RNA polymerase. Nature 2011 Oct 478 (7368)