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Goat anti-carbonic anhydrase XII (aa188-199) Antibody
Item#:
dAP-3027
Size Price Quantity
50.0 ug $310.00
Goat anti-carbonic anhydrase XII (aa188-199) Antibody
Principle Name carbonic anhydrase XII (aa188-199)
Official Symbol CA12
All Name and Symbols CA12; carbonic anhydrase XII; CAXII; HsT18816; CA-XII; carbonate dehydratase XII; carbonic anhydrase 12; carbonic dehydratase; tumor antigen HOM-RCC-3.1.3
Accession Number(s) NP_001209.1; NP_996808.1; NP_001280571.1
Peptide Sequence SHLQHVKYKGQE, is from internal region
Human Gene ID(s) 771
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10µg/ml.
Reference(s) Muhammad E et al. Autosomal recessive hyponatremia due to isolated salt wasting in sweat associated with a mutation in the active site of Carbonic Anhydrase 12. Hum Genet. 2011 Apr;129(4)

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