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Goat anti-NR5A2 / LRH1 Antibody
Item#:
dAP-3021
Size Price Quantity
50.0 ug $310.00
Goat anti-NR5A2 / LRH1 Antibody
Principle Name NR5A2 / LRH1
Official Symbol NR5A2
All Name and Symbols NR5A2; nuclear receptor subfamily 5, group A, member 2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F-2; CYP7A promoter-binding factor; alpha-1-fetoprotein transcription factor; b1-binding factor, hepatocyte transcription factor which activat
Accession Number(s) NP_995582.1; NP_003813.1
Peptide Sequence TDYDRSPFVTSPIS, is from internal region
Human Gene ID(s) 2494
Non-Human GeneID(s) 26424 (mouse); 60349 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human, Pig
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration
Reference(s) Yumoto F, Nguyen P, Sablin EP, Baxter JD, Webb P, Fletterick RJ. Structural basis of coactivation of liver receptor homolog-1 by ß-catenin. Proc Natl Acad Sci U S A. 2012 Jan 3;109(1)

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