Goat anti-MAP2K2 / MEK2 Antibody

Item#:

dAP-3000

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MAP2K2 / MEK2 Antibody
Principle Name	MAP2K2 / MEK2
Official Symbol	MAP2K2
All Name and Symbols	MAP2K2; mitogen-activated protein kinase kinase 2; MAPKK2; MEK2; MKK2; PRKMK2; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 2; dual specificity mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45
Accession Number(s)	NP_109587.1
Peptide Sequence	DLKMLTNHTFIKRS, is from internal region
Human Gene ID(s)	5605
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 48kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kDa according to NP_109587.1). Recommended concentration
Reference(s)	Duhamel S, Hébert J, Gaboury L, Bouchard A, Simon R, Sauter G, Basik M, Meloche S. Sef downregulation by Ras causes MEK1/2 to become aberrantly nuclear localized leading to polyploidy and neoplastic transformation. Cancer Res. 2012 Feb 1;72(3)