Goat anti-PHF11 Antibody |
| Principle Name | PHF11 |
| Official Symbol | PHF11 |
| All Name and Symbols | PHF11; PHD finger protein 11; APY; BCAP; IGEL; IGER; IGHER; NY-REN-34; NYREN34; RP11-185C18.3; IgE responsiveness (atopic); NY-REN-34 antigen |
| Accession Number(s) | NP_001035533.1; NP_001035534.1 |
| Peptide Sequence | KIHASQQRWQQLKE, is from internal region |
| Human Gene ID(s) | 51131 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001035533.1; NP_001035534.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 37kDa band observed in nuclear lysates of cell lines Daudi, Jurkat and Jurkat (calculated MW of 37.6kDa according to NP_001035533.1). Recommended concentration |
| Reference(s) | Zhang Y, Leaves NI, Anderson GG, Ponting CP, Broxholme J, Holt R, Edser P, Bhattacharyya S, Dunham A, Adcock IM, Pulleyn L, Barnes PJ, Harper JI, Abecasis G, Cardon L, White M, Burton J, Matthews L, Mott R, Ross M, Cox R, Moffatt MF, Cookson WO Positional cloning of a quantitative trait locus on chromosome 13q14 that influences immunoglobulin E levels and asthma Nat Genet. 2003 Jun;34(2) |
