Goat anti-EDF1 Antibody |
| Principle Name | EDF1 |
| Official Symbol | EDF1 |
| All Name and Symbols | EDF1; endothelial differentiation-related factor 1; EDF-1; MBF1; multiprotein bridging factor 1 |
| Accession Number(s) | NP_003783.1; NP_694880.1 |
| Peptide Sequence | NKQHSITKNTAKLDR, is from internal region |
| Human Gene ID(s) | 8721 |
| Non-Human GeneID(s) | 59022 (mouse); 678956 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Brendel C, Gelman L, Auwerx J. Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism. Molecular endocrinology (Baltimore, Md.) 2002 Jun 16 (6) |
