Goat anti-Transcription factor E2F1 (aa314-327) Antibody

Item#:

dAP-2975

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Transcription factor E2F1 (aa314-327) Antibody
Principle Name	Transcription factor E2F1 (aa314-327)
Official Symbol	E2F1
All Name and Symbols	E2F1; E2F transcription factor 1; E2F-1; RBAP1; RBBP3; RBP3; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; transcription factor E2F1
Accession Number(s)	NP_005216.1
Peptide Sequence	QEVTSEEENRATDS, is from internal region
Human Gene ID(s)	1869
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9kDa according to NP_005216.1). Recommended concentration
Reference(s)	Hu F, Gartenhaus RB, Zhao XF, Fang HB, Minkove S, Poss DE, Rapoport AP. c-Myc and E2F1 drive PBK/TOPK expression in high-grade malignant lymphomas. Leukemia research 2013 Apr 37 (4)