Goat anti-Transcription factor E2F4 Antibody

Item#:

dAP-2973

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Transcription factor E2F4 Antibody
Principle Name	Transcription factor E2F4
Official Symbol	E2F4
All Name and Symbols	E2F4; E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; transcription factor E2F4
Accession Number(s)	NP_001941.2
Peptide Sequence	PKELSEIFDPTR, is from internal region
Human Gene ID(s)	1874
Non-Human GeneID(s)	104394 (mouse); 100360427 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Pig
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 50kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0kDa according to NP_001941.2). Recommended concentration
Reference(s)	Beshiri ML, Holmes KB, Richter WF, Hess S, Islam AB, Yan Q, Plante L, Litovchick L, Gévry N, Lopez-Bigas N, Kaelin WG, Benevolenskaya EV. Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation. Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 109 (45)