Goat anti-SPO11 Antibody |
| Principle Name | SPO11 |
| Official Symbol | SPO11 |
| All Name and Symbols | SPO11; SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae); MGC39953; SPO11 meiotic protein covalently bound to DSB-like; SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like; meiotic recombination protein SPO11 |
| Accession Number(s) | NP_036576.1; NP_937998.1 |
| Peptide Sequence | NTYATKRDIYYTDSQ, is from internal region |
| Human Gene ID(s) | 23626 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_036576.1; NP_937998.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 38kDa band observed in Human Testis lysates (calculated MW of 40.4kDa according to NP_937998.1). Recommended concentration |
| Reference(s) | Buhler C, Borde V, Lichten M Mapping meiotic single-strand DNA reveals a new landscape of DNA double-strand breaks in Saccharomyces cerevisiae PLoS Biol. 2007 Dec;5(12) |
