Goat anti-ITK Antibody |
| Principle Name | ITK |
| Official Symbol | ITK |
| All Name and Symbols | ITK; IL2-inducible T-cell kinase; EMT; LYK; MGC126257; MGC126258; PSCTK2; homolog of mouse T-cell itk/tsk; tyrosine-protein kinase ITK/TSK; tyrosine-protein kinase LYK |
| Accession Number(s) | NP_005537.3 |
| Peptide Sequence | KERPEDRPAFSR, is from internal (near C terminus) |
| Human Gene ID(s) | 3702 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 70kDa band observed in Rat Thymus lysates (calculated MW of 71.8kDa according to NP_005537.3). Recommended concentration |
| Reference(s) | Hu J, Sahu N, Walsh E, August A Memory phenotype CD8+ T cells with innate function selectively develop in the absence of active Itk Eur J Immunol. 2007 Oct;37(10) |
