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Goat anti-Caspase 6 (alpha) Antibody


Item#:
dAP-2939
Size Price Quantity
50.0 ug $310.00
Goat anti-Caspase 6 (alpha) Antibody
Principle Name Caspase 6 (alpha)
Official Symbol CASP6
All Name and Symbols CASP6; caspase 6, apoptosis-related cysteine peptidase; MCH2; apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine protease; caspase-6
Accession Number(s) NP_001217.2; NP_116787.1
Peptide Sequence NRKVSQRRVDFCKDP, is from internal region
Human Gene ID(s) 839
Non-Human GeneID(s) 12368 (mouse); 83584 (rat);
Important Comments This antibody is expected to recognize reproted isoform alpha (NP_001217.2) only. The immunizing peptide overlaps phosphorylated serine 257 and therefore this antibody is not expected to recognize the phosphorylated protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 33kDa band observed in Human Colon lysates (calculated MW of 33.3kDa according to NP_001217.2). Recommended concentration
Reference(s) Stanger K, Steffek M, Zhou L, Pozniak CD, Quan C, Franke Y, Tom J, Tam C, Elliott JM, Lewcock JW, Zhang Y, Murray J, Hannoush RN. Allosteric peptides bind a caspase zymogen and mediate caspase tetramerization. Nature chemical biology 2012 Jul 8 (7)

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