Goat anti-CBFB Antibody |
| Principle Name | CBFB |
| Official Symbol | CBFB |
| All Name and Symbols | CBFB; core-binding factor, beta subunit; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; core-binding factor subunit beta; polyomavirus enhancer |
| Accession Number(s) | NP_074036.1; NP_001746.1 |
| Peptide Sequence | EARRQQDPSPGSN, is from internal region (near C Terminus) |
| Human Gene ID(s) | 865 |
| Non-Human GeneID(s) | 12400 (mouse); |
| Important Comments | This antibody is expected to recognize reported isoform 1 (NP_074036.1) only, however it is expected to recognize reported mouse isoforms 1 (NP_071704.3), 2 (NP_001154928.1) and 3 (NP_001154929.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx 22kDa band observed in lysates of cell line Daudi (calculated MW of 22.0kDa according to NP_074036.1). Recommended concentration |
| Reference(s) | Hultquist JF, Binka M, LaRue RS, Simon V, Harris RS. Vif proteins of human and simian immunodeficiency viruses require cellular CBFß to degrade APOBEC3 restriction factors. Journal of virology 2012 Mar 86 (5) |
