Goat anti-BCAR1 Antibody |
| Principle Name | BCAR1 |
| Official Symbol | BCAR1 |
| All Name and Symbols | BCAR1; breast cancer anti-estrogen resistance 1; CAS; CAS1; CASS1; CRKAS; P130Cas; Cas scaffolding protein family member 1; Crk-associated substrate p130Cas; breast cancer anti-estrogen resistance protein 1 |
| Accession Number(s) | NP_001164185.1; NP_001164186.1; NP_001164187.1; NP_001164188.1; NP_001164189.1; NP_055382.2; NP_001164190.1; NP_001164191.1; NP_001164192.1 |
| Peptide Sequence | KQTSTFSKQTPHH, is from internal region |
| Human Gene ID(s) | 9564 |
| Non-Human GeneID(s) | 12927 (mouse); 25414 (rat); |
| Important Comments | This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Huang W, Deng B, Wang RW, Tan QY, He Y, Jiang YG, Zhou JH. BCAR1 protein plays important roles in carcinogenesis and predicts poor prognosis in non-small-cell lung cancer. PloS one 2012 7 (4) |
