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Goat anti-HIC1 (aa396-410) Antibody
Item#:
dAP-2920
Size Price Quantity
50.0 ug $310.00
Goat anti-HIC1 (aa396-410) Antibody
Principle Name HIC1 (aa396-410)
Official Symbol HIC1
All Name and Symbols HIC1; hypermethylated in cancer 1; ZBTB29; ZNF901; hic-1; hypermethylated in cancer 1 protein; zinc finger and BTB domain-containing protein 29
Accession Number(s) NP_006488.2; NP_001091672.1
Peptide Sequence HLEGYPCPHLAYGEP, is from internal region
Human Gene ID(s) 3090
Non-Human GeneID(s) 15248 (mouse); 303310 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB Trf
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated
Reference(s) Yu J, Liu P, Cui X, Sui Y, Ji G, Guan R, Sun D, Ji W, Liu F, Liu A, Zhao Y, Yu Y, Jin Y, Bai J, Geng J, Xue Y, Qi J, Lee KY, Fu S. Identification of novel subregions of LOH in gastric cancer and analysis of the HIC1 and TOB1 tumor suppressor genes in these subregions. Mol Cells. 2011 Jul;32(1)

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