Goat anti-MC4R Antibody |
| Principle Name | MC4R |
| Official Symbol | MC4R |
| All Name and Symbols | MC4R; melanocortin 4 receptor; MC4-R; melanocortin receptor 4 |
| Accession Number(s) | NP_005903.2 |
| Peptide Sequence | NSTHRGMHTSLH, is from N Terminus |
| Human Gene ID(s) | 4160 |
| Non-Human GeneID(s) | |
| Important Comments | The immunizing peptide represents extracellular part of the protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments in Human Brain, Colon, Duodenum, Ileum and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any re |
| Reference(s) | McDaniel FK, Molden BM, Mohammad S, Baldini G, McPike L, Narducci P, Granell S, Baldini G. Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) is essential to maintain receptor responsiveness to a-melanocyte-stimulating hormone (a-MSH). The Journal of biological chemistry 2012 Jun 287 (26) |
