Goat anti-NPC1 Antibody |
| Principle Name | NPC1 |
| Official Symbol | NPC1 |
| All Name and Symbols | NPC1; Niemann-Pick disease, type C1; FLJ98532; NPC |
| Accession Number(s) | NP_000262.2 |
| Peptide Sequence | TYHTVLQTSADFID, is from internal region |
| Human Gene ID(s) | 4864 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 170kDa band observed in lysates of cell line HEK293 (calculated MW of 142kDa according to NP_000262.2). Recommended concentration |
| Reference(s) | Tang Y, Leao IC, Coleman EM, Broughton RS, Hildreth JE, Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments. Journal of virology 2009 Aug 83 (16) |
