Goat anti-PIM2 Antibody |
| Principle Name | PIM2 |
| Official Symbol | PIM2 |
| All Name and Symbols | PIM2; pim-2 oncogene; pim-2h; proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2; serine/threonine-protein kinase pim-2 |
| Accession Number(s) | NP_006866.2 |
| Peptide Sequence | QTPAEDVPLNPSK, is from C Terminus |
| Human Gene ID(s) | 11040 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB Trf |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended con |
| Reference(s) | Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N, Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels. Cancer research 2008 Jul 68 (13) |