Goat anti-PRKCB Antibody |
| Principle Name | PRKCB |
| Official Symbol | PRKCB |
| All Name and Symbols | PRKCB; protein kinase C, beta; MGC41878; PKC-beta; PKCB; PRKCB1; PRKCB2; OTTHUMP00000162377; OTTHUMP00000162378; PKC-B; protein kinase C beta type; protein kinase C, beta 1 polypeptide |
| Accession Number(s) | NP_997700.1 |
| Peptide Sequence | DKEFTRQPVELT, is from internal region (near C terminus) |
| Human Gene ID(s) | 5579 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_997700.1) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2µg/ml. |
| Reference(s) | Sheng YJ, Gao JP, Li J, Han JW, Xu Q, Hu WL, Pan TM, Cheng YL, Yu ZY, Ni C, Yao S, He CF, Liu YS, Li Y, Ge HM, Xiao FL, Sun LD, Yang S, Zhang XJ. Follow-up study identifies two novel susceptibility loci PRKCB and 8p11.21 for systemic lupus erythematosus. Rheumatology (Oxford). 2011 Apr;50(4) |
