Goat anti-Pycard (mouse) Antibody

Item#:

dAP-2857

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Pycard (mouse) Antibody
Principle Name	Pycard (mouse)
Official Symbol	Pycard
All Name and Symbols	Pycard; PYD and CARD domain containing; 9130417A21Rik; Asc; CARD5; TMS-1; TNS1; masc; PYD and CARD domain-containing protein; apoptosis-associated speck-like protein containing a CARD
Accession Number(s)	NP_075747.3
Peptide Sequence	SVPAQSTARTGH, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)	66824 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Preliminary experiments in Mouse Spleen, Lymph Node and Thymus lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been re
Reference(s)	Compan V, Baroja-Mazo A, López-Castejón G, Gomez AI, Martínez CM, Angosto D, Montero MT, Herranz AS, Bazán E, Reimers D, Mulero V, Pelegrín P. Cell Volume Regulation Modulates NLRP3 Inflammasome Activation. Immunity 2012 Sep 37 (3)