Goat anti-TNNT3 Antibody |
| Principle Name | TNNT3 |
| Official Symbol | TNNT3 |
| All Name and Symbols | TNNT3; troponin T type 3 (skeletal, fast); TNTF; beta TnTF; beta-TnTF; fTnT; fast skeletal muscle troponin T; troponin T, fast skeletal muscle; troponin-T3, skeletal, fast |
| Accession Number(s) | NP_006748.1; NP_001036246.1; NP_001036247.1 |
| Peptide Sequence | TTLRSRIDQAQKHSK, is from C Terminus |
| Human Gene ID(s) | 7140 |
| Non-Human GeneID(s) | 21957 (mouse); 24838 (rat); |
| Important Comments | This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et |
| Immunohistochemistry | In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8µg/ml. |
| Reference(s) | Zhao N, Jiang M, Han W, Bian C, Li X, Huang F, Kong Q, Li J. A novel mutation in TNNT3 associated with Sheldon-Hall syndrome in a Chinese family with vertical talus. Eur J Med Genet. 2011 May-Jun;54(3) |
