Goat anti-creatine kinase M-type Antibody

Item#:

dAP-2817

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-creatine kinase M-type Antibody
Principle Name	creatine kinase M-type
Official Symbol	CKM
All Name and Symbols	CKM; creatine kinase, muscle; CKMM; M-CK; creatine kinase M chain; creatine kinase M-type; creatine kinase-M
Accession Number(s)	NP_001815.2
Peptide Sequence	NTHNKFKLNYKPEE, is from N Terminus
Human Gene ID(s)	1158
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration
Reference(s)	Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M. Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy. J Biol Chem. 2011 Oct 7;286(40)