Goat anti-RFX5 Antibody |
| Principle Name | RFX5 |
| Official Symbol | RFX5 |
| All Name and Symbols | RFX5; regulatory factor X, 5 (influences HLA class II expression); OTTHUMP00000196318; regulatory factor X, 5 |
| Accession Number(s) | NP_000440.1 |
| Peptide Sequence | TGDKSSEPSTLSNE, is from internal region |
| Human Gene ID(s) | 5993 |
| Non-Human GeneID(s) | 53970 (mouse); 310659 (rat); |
| Important Comments | Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration |
| Reference(s) | Kong X, Fang M, Li P, Fang F, Xu Y, HDAC2 deacetylates class II transactivator and suppresses its activity in macrophages and smooth muscle cells. Journal of molecular and cellular cardiology 2009 Mar 46 (3) |
