Goat anti-biotinidase (aa372-385) Antibody

Item#:

dAP-2790

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-biotinidase (aa372-385) Antibody
Principle Name	biotinidase (aa372-385)
Official Symbol	BTD
All Name and Symbols	BTD; biotinidase; OTTHUMP00000160300; OTTHUMP00000209134; OTTHUMP00000209135; OTTHUMP00000209138; biotinase
Accession Number(s)	NP_000051.1
Peptide Sequence	EKDAQEVHCDEATK, is from internal region
Human Gene ID(s)	686
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.1kDa according to NP_000051.1). Recommended concentration
Reference(s)	Cowan TM, Blitzer MG, Wolf B; Working Group of the American College of Medical Genetics Laboratory Quality Assurance Committee. Technical standards and guidelines for the diagnosis of biotinidase deficiency. Genet Med. 2010 Jul;12(7)