Goat anti-myotilin (aa209-221) Antibody

Item#:

dAP-2768

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-myotilin (aa209-221) Antibody
Principle Name	myotilin (aa209-221)
Official Symbol	MYOT
All Name and Symbols	MYOT; myotilin; LGMD1; LGMD1A; TTID; 57 kDa cytoskeletal protein; OTTHUMP00000159430; OTTHUMP00000223460; OTTHUMP00000223461; myofibrillar titin-like Ig domains protein; titin immunoglobulin domain protein (myotilin)
Accession Number(s)	NP_006781.1; NP_001129412.1
Peptide Sequence	DSQQHNSEHARLQ, is from internal region
Human Gene ID(s)	9499
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize both reported isoforms (NP_006781.1; NP_001129412.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 55.4kDa according to NP_006781.1. Recommended concentration
Reference(s)	von Nandelstadh P, Soliymani R, Baumann M, Carpen O. Analysis of myotilin turnover provides mechanistic insight into the role of myotilinopathy-causing mutations. Biochem J. 2011 May 15;436(1)