Goat anti-ENC1 Antibody |
| Principle Name | ENC1 |
| Official Symbol | ENC1 |
| All Name and Symbols | ENC1; ectodermal-neural cortex 1 (with BTB-like domain); CCL28; ENC-1; FLJ39259; KLHL35; KLHL37; NRPB; PIG10; TP53I10; OTTHUMP00000128235; OTTHUMP00000222054; ectoderm-neural cortex protein 1; ectodermal-neural cortex (with BTB-like domain); kelch-like 35 |
| Accession Number(s) | NP_003624.1; NP_001243505.1 |
| Peptide Sequence | TSVSHDKLPKVQC, is from internal region |
| Human Gene ID(s) | 8507 |
| Non-Human GeneID(s) | 13803 (mouse); 294674 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_003624.1; NP_001243505.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Rat, Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Adrenal Gland. Recommended concentration |
| Reference(s) | Wang XJ, Zhang DD. Ectodermal-neural cortex 1 down-regulates Nrf2 at the translational level. PLoS One. 2009;4(5) |