Goat anti-ICAM1 Antibody |
| Principle Name | ICAM1 |
| Official Symbol | ICAM1 |
| All Name and Symbols | ICAM1; intercellular adhesion molecule 1; BB2; CD54; P3.58; ICAM-1; cell surface glycoprotein P3.58; human rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor |
| Accession Number(s) | NP_000192.2 |
| Peptide Sequence | SNCPDGQSTAKT, is from internal region |
| Human Gene ID(s) | 3383 |
| Non-Human GeneID(s) | |
| Important Comments | The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1). The observed molecular weight corresponds to earlier findings |
| Reference(s) | Fu C, Tong C, Wang M, Gao Y, Zhang Y, Lü S, Liang S, Dong C, Long M. Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay. J Biol Chem. 2011 Oct 7;286(40) |
