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Goat anti-calbindin D28 Antibody
Item#:
dAP-2729
Size Price Quantity
50.0 ug $310.00
Goat anti-calbindin D28 Antibody
Principle Name calbindin D28
Official Symbol CALB1
All Name and Symbols CALB1; calbindin 1, 28kDa; CALB; D-28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL-H protein; calbindin; calbindin 1, (28kD); calbindin D28; vitamin D-dependent calcium-binding protein, avian-type
Accession Number(s) NP_004920.1
Peptide Sequence KTFVDQYGQRDDGK, is from internal region
Human Gene ID(s) 793
Non-Human GeneID(s) 83839 (rat);
Important Comments Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with di
Immunohistochemistry Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration, 0.1-0.3µg/ml.
Reference(s) Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y. DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis. J Reprod Immunol. 2010 Jun;85(2)

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