Goat anti-PCNA Antibody |
| Principle Name | PCNA |
| Official Symbol | PCNA |
| All Name and Symbols | PCNA; proliferating cell nuclear antigen; MGC8367; DNA polymerase delta auxiliary protein; OTTHUMP00000030189; OTTHUMP00000030190; cyclin |
| Accession Number(s) | NP_002583.1 |
| Peptide Sequence | NGNIKLSQTSNVD, is from internal region |
| Human Gene ID(s) | 5111 |
| Non-Human GeneID(s) | 18538 (mouse); 25737 (rat); |
| Important Comments | Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, EIA |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW |
| Reference(s) | Jones MJ, Colnaghi L, Huang TT. Dysregulation of DNA polymerase ¿ recruitment to replication forks results in genomic instability. EMBO J. 2011 Dec 13..PMID |
