Goat anti-PRMT2 Antibody |
| Principle Name | PRMT2 |
| Official Symbol | PRMT2 |
| All Name and Symbols | PRMT2; protein arginine methyltransferase 2; HRMT1L1; MGC111373; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1; HMT1 hnRNP methyltransferase-like 1; OTTHUMP00000046525; OTTHUMP00000046526; OTTHUMP00000115881; OTTHUMP00000115882; OTTHUMP00000115884; |
| Accession Number(s) | NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1 |
| Peptide Sequence | DQPRTTKYHSVILQ, is from internal region |
| Human Gene ID(s) | 3275 |
| Non-Human GeneID(s) | 15468 (mouse); 499420 (rat); |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 37kDa band observed in lysates of cell line HeLa and in rodent Brain lysates (calculated MW of 37.2kDa according to NP_001229793.1). Approx 35kDa band observed in Pig Brain lysates (calculated MW of 38.7kDa according to XP_005658963. |
| Reference(s) | Pak ML, Lakowski TM, Thomas D, Vhuiyan MI, Hüsecken K, Frankel A. A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity. Biochemistry. 2011 Sep 27;50(38) |