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Goat anti-complement C3 (aa681-693) Antibody
Item#:
dAP-2707
Size Price Quantity
50.0 ug $310.00
Goat anti-complement C3 (aa681-693) Antibody
Principle Name complement C3 (aa681-693)
Official Symbol C3
All Name and Symbols C3; complement component 3; AHUS5; ARMD9; ASP; CPAMD1; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; OTTHUMP00000197086; acylation-stimulating protein cleavage product; complement C3; complement component C3
Accession Number(s) NP_000055.2
Peptide Sequence DKVGKYPKELRKC, is from internal region
Human Gene ID(s) 718
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize the C3 alpha chain and C3a anaphylatoxin,
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104k
Reference(s) Kenyon KD, Cole C, Crawford F, Kappler JW, Thurman JM, Bratton DL, Boackle SA, Henson PM. IgG autoantibodies against deposited C3 inhibit macrophage-mediated apoptotic cell engulfment in systemic autoimmunity. J Immunol. 2011 Sep 1;187(5)

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