Goat anti-TUBB3 Antibody |
| Principle Name | TUBB3 |
| Official Symbol | TUBB3 |
| All Name and Symbols | TUBB3; tubulin, beta 3; CDCBM; CFEOM3A; TUBB4; beta-4; OTTHUMP00000176846; class III beta-tubulin; tubulin beta-3 chain; tubulin beta-4 chain; tubulin beta-III |
| Accession Number(s) | NP_006077.2 |
| Peptide Sequence | DPSGNYVGDSD, is from internal region (near N terminus) |
| Human Gene ID(s) | 10381 |
| Non-Human GeneID(s) | 22152 (mouse); 246118 (rat); |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_006077.2) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 52kDa band observed in lysates of cell line HepG2 (calculated MW of 50.4kDa according to NP_006077.2). Recommended concentration |
| Reference(s) | Hetland TE, Hellesylt E, Flørenes VA, Tropé C, Davidson B, Kærn J. Class III ß-tubulin expression in advanced-stage serous ovarian carcinoma effusions is associated with poor survival and primary chemoresistance. Hum Pathol. 2011 Jul;42(7) |
