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Goat anti-Unc5b (mouse, rat) Antibody
Item#:
dAP-2704
Size Price Quantity
50.0 ug $310.00
Goat anti-Unc5b (mouse, rat) Antibody
Principle Name Unc5b (mouse, rat)
Official Symbol Unc5b
All Name and Symbols Unc5b; unc-5 homolog B (C. elegans); 6330415E02Rik; A630020F16; D10Bwg0792e; Unc5h2; netrin receptor UNC5B; protein unc-5 homolog 2; protein unc-5 homolog B; unc-5 homolog 2; unc5 homolog 2
Accession Number(s) NP_084046.2
Peptide Sequence DWIFQLKTQAHQGH, is from internal region
Human Gene ID(s) 219699
Non-Human GeneID(s) 107449 (mouse); 60630 (rat);
Important Comments This antibody is expected to recognize the cytoplasmic domain of the protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep_ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments in Human and rodent Brain, Lung and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results be
Reference(s) Koch AW, Mathivet T, Larrivée B, Tong RK, Kowalski J, Pibouin-Fragner L, Bouvrée K, Stawicki S, Nicholes K, Rathore N, Scales SJ, Luis E, del Toro R, Freitas C, Bréant C, Michaud A, Corvol P, Thomas JL, Wu Y, Peale F, Watts RJ, Tessier-Lavigne M, Bagri A, Robo4 maintains vessel integrity and inhibits angiogenesis by interacting with UNC5B. Dev Cell. 2011 Jan 18;20(1)

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