Goat anti-PDCD4 Antibody |
| Principle Name | PDCD4 |
| Official Symbol | PDCD4 |
| All Name and Symbols | PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); H731; MGC33046; MGC33047; OTTHUMP00000020483; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death protein 4; protein 197/15a |
| Accession Number(s) | NP_055271.2; NP_663314.1; NP_001186421.1 |
| Peptide Sequence | DSYKGTVDCVQAR, is from internal region |
| Human Gene ID(s) | 27250 |
| Non-Human GeneID(s) | 18569 (mouse); 64031 (rat); |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 60kDa band observed in nuclear lysates of cell lines NIH3T3 and HeLa (calculated MW of 51.7kDa according to NP_055271.2). Recommended concentration |
| Reference(s) | Zhang X, Wang X, Song X, Liu C, Shi Y, Wang Y, Afonja O, Ma C, Chen YH, Zhang L. Programmed cell death 4 enhances chemosensitivity of ovarian cancer cells by activating death receptor pathway in vitro and in vivo. Cancer Sci. 2010 Oct;101(10) |
