Goat anti-Fibrillarin / FBL Antibody

Item#:

dAP-2677

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Fibrillarin / FBL Antibody
Principle Name	Fibrillarin / FBL
Official Symbol	FBL
All Name and Symbols	FBL; fibrillarin; FIB; FLRN; RNU3IP1; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1; rRNA 2'-O-methyltransferase fibrillarin
Accession Number(s)	NP_001427.2
Peptide Sequence	KRVSISEGDDKIEYR, is from internal region
Human Gene ID(s)	2091
Non-Human GeneID(s)	14113 (mouse); 292747 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8kDa according to NP_001427.2). Recommended concentration
Reference(s)	Padilla PI, Uhart M, Pacheco-Rodriguez G, Peculis BA, Moss J, Vaughan M. Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9)