Goat anti-ARF4 (aa137-150) Antibody

Item#:

dAP-2672

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ARF4 (aa137-150) Antibody
Principle Name	ARF4 (aa137-150)
Official Symbol	ARF4
All Name and Symbols	ARF4; ADP-ribosylation factor 4; ARF2; ADP-ribosylation factor 2; OTTHUMP00000213140; OTTHUMP00000213142; OTTHUMP00000213211
Accession Number(s)	NP_001651.1
Peptide Sequence	SEMTDKLGLQSLRN, is from internal region
Human Gene ID(s)	378
Non-Human GeneID(s)	11843 (mouse); 79120 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to NP_001651.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Thymus. Recommended concentration
Reference(s)	Jang SY, Jang SW, Ko J. Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration. Cancer Lett. 2012 Jan 28;314(2)