Goat anti-Ryk (mouse) Antibody

Item#:

dAP-2653

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Ryk (mouse) Antibody
Principle Name	Ryk (mouse)
Official Symbol	Ryk
All Name and Symbols	Ryk; receptor-like tyrosine kinase; AW536699; ERK-3; Vik; NYK-R; growth factor receptor; kinase VIK; met-related kinase; tyrosine-protein kinase RYK
Accession Number(s)	NP_038677.3; NP_001036072.1
Peptide Sequence	HAKSKVEYKLGFQ, is from internal region
Human Gene ID(s)	6259
Non-Human GeneID(s)	20187 (mouse); 140585 (rat);
Important Comments	This antibody is expected to recognize both reported isoforms (NP_038677.3; NP_001036072.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave an approx 85kDa band in Mouse Lung lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.3kD
Reference(s)	Berndt JD, Aoyagi A, Yang P, Anastas JN, Tang L, Moon RT. Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/ß-catenin signaling. J Cell Biol. 2011 Sep 5;194(5)