Goat anti-Gelsolin (isoform a) Antibody

Item#:

dAP-2646

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Gelsolin (isoform a) Antibody
Principle Name	Gelsolin (isoform a)
Official Symbol	GSN
All Name and Symbols	GSN; gelsolin; ADF; AGEL; DKFZp313L0718; OTTHUMP00000022011; OTTHUMP00000022014; OTTHUMP00000162202; actin-depolymerizing factor; brevin
Accession Number(s)	NP_000168.1; NP_937895.1; NP_001121138.1
Peptide Sequence	PQGRVPEARPNS, is from N Terminus
Human Gene ID(s)	2934
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize isoform a (NP_000168.1) only . Reported variants represent identical protein
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 100kDa band observed in Human Colon, Duodenum, Heart and Skeletal Muscle lysates (calculated MW of 85.7kDa according to NP_000168.1). Recommended concentration
Reference(s)	An JH, Kim JW, Jang SM, Kim CH, Kang EJ, Choi KH. Gelsolin negatively regulates the activity of tumor suppressor p53 through their physical interaction in hepatocarcinoma HepG2 cells. Biochem Biophys Res Commun. 2011 Aug 19;412(1)