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Goat anti-Praja-1 / RNF70 (aa285-298) Antibody
Item#:
dAP-2642
Size Price Quantity
50.0 ug $310.00
Goat anti-Praja-1 / RNF70 (aa285-298) Antibody
Principle Name Praja-1 / RNF70 (aa285-298)
Official Symbol PJA1
All Name and Symbols PJA1; praja ring finger 1; PRAJA1; RNF70; E3 ubiquitin-protein ligase Praja-1; OTTHUMP00000023457; OTTHUMP00000023458; OTTHUMP00000158771; RING finger protein 70; praja 1
Accession Number(s) NP_660095.1; NP_001027568.1; NP_071763.2
Peptide Sequence HSTSRWRDTANDNE, is from internal region
Human Gene ID(s) 64219
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all reported isoforms (NP_660095.1; NP_001027568.1; NP_071763.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments gave an approx 30kDa band in Human Brain and Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated
Reference(s) Zoabi M, Sadeh R, de Bie P, Marquez VE, Ciechanover A. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem Biophys Res Commun. 2011 May 13;408(3)

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