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Goat anti-HMHA1 (aa57-71) Antibody
Item#:
dAP-2620
Size Price Quantity
50.0 ug $310.00
Goat anti-HMHA1 (aa57-71) Antibody
Principle Name HMHA1 (aa57-71)
Official Symbol HMHA1
All Name and Symbols HMHA1; histocompatibility (minor) HA-1; HA-1; HLA-HA1; KIAA0223; minor histocompatibility antigen HA-1; minor histocompatibility protein HA-1
Accession Number(s) NP_036424.2
Peptide Sequence KATGTLKRPTSLSRH, is from N Terminus
Human Gene ID(s) 23526
Non-Human GeneID(s) 70719 (mouse); 314618 (rat);
Important Comments To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments gave an approx 200kDa band in lysates of cell lines Jurkat and Molt4 after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the cal
Reference(s) Nicholls S, Piper KP, Mohammed F, Dafforn TR, Tenzer S, Salim M, Mahendra P, Craddock C, van Endert P, Schild H, Cobbold M, Engelhard VH, Moss PA, Willcox BE. Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10)

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