Goat anti-ZAP70 Antibody |
Principle Name | ZAP70 |
Official Symbol | ZAP70 |
All Name and Symbols | ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; FLJ17670; FLJ17679; SRK; STD; TZK; ZAP-70; 70 kDa zeta-associated protein; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase, 70kD |
Accession Number(s) | NP_001070.2 |
Peptide Sequence | SDGYTPEPARITSPD, is from internal region |
Human Gene ID(s) | 7535 |
Non-Human GeneID(s) | 22637 (mouse); 301348 (rat); |
Important Comments | This antibody is expected to recognize isoform 1 (NP_001070.2) only. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB, IP |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 32000. |
Western Blot | Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration |
Reference(s) | Crespo M, Bosch F, Villamor N, Bellosillo B, Colomer D, Rozman M, Marcé S, López-Guillermo A, Campo E, Montserrat E. ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med. 2003 May 1;348(18) |