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Goat anti-ZAP70 Antibody


Item#:
dAP-2616
Size Price Quantity
50.0 ug $310.00
Goat anti-ZAP70 Antibody
Principle Name ZAP70
Official Symbol ZAP70
All Name and Symbols ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; FLJ17670; FLJ17679; SRK; STD; TZK; ZAP-70; 70 kDa zeta-associated protein; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase, 70kD
Accession Number(s) NP_001070.2
Peptide Sequence SDGYTPEPARITSPD, is from internal region
Human Gene ID(s) 7535
Non-Human GeneID(s) 22637 (mouse); 301348 (rat);
Important Comments This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IP
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration
Reference(s) Crespo M, Bosch F, Villamor N, Bellosillo B, Colomer D, Rozman M, Marcé S, López-Guillermo A, Campo E, Montserrat E. ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med. 2003 May 1;348(18)

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